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KMID : 0861020170320030045
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Korea Journal of Herbology
2017 Volume.32 No. 3 p.45 ~ p.53
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Inhibition Effects on Oxidative DNA Damage and Anti-inflammatory Effects of Nelumbinis Flos
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Jeong Hyung-Jin
Park Yeon-Gyeong
Jang Tae-Won
Kim Do-Wan
Jeong Jin-Boo
Park Jae-Ho
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Abstract
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Objective : Nelumbo nucifera , its rhizome and semen have been used as a traditional medicine which was studied on antioxidant, hepatoprotective effect, anti-obesity and the others. However, Nelumbinis Flos have not studied. We investigated protective effects on oxidative DNA damage and anti-inflammatory effects of Nelumbinis Flos.
Methods : The antioxidant activity was conducted by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2`-Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay and reducing power assay. Total phenolic content was analyzed. Also, phenolic compounds were detected by HPLC/UV. The inhibitory effect on oxidative DNA damage was determined using ΦX-174 RF I plasmid DNA cleavage assay. The anti-inflammatory effect of Nelumbinis Flos was measured by the amount of nitric-oxide (NO) produced and protein levels of iNOS, and COX-2 in LPS induced RAW 264.7 cells.
Results : The results of DPPH and ABTS radical scavenging activity at 200 ㎍/㎖ of extraction were 97.02±0.88% and 96.42±0.25%. Reducing power (fold of L-ascorbic acid as control) was 100.14±0.31 at 200 ㎍/㎖. Total phenol content was 8.70±0.02 ㎎/g. Chlorogenic acid, catechin and epicatechin were found by HPLC. Nelumbinis Flos has inhibitory effect in dose-manner against oxidative DNA damage. In addition, it showed the anti-inflammatory effect by suppression of NO production as well as protein levels of iNOS, and COX-2.
Conclusion : This study suggested that Nelumbinis Flos showed potential antioxidant and suppression activities of various factors were related in NO produced. Therefore, Nelumbinis Flos as natural plant resources that may help reduce inflammation and alleviate DNA damage.
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KEYWORD
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Anti-inflammatory effect, Antioxidant, DNA damage, Nelumbinis Flos
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